dCas9-KRAB overexpressing cell line

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The dCas9-KRAB overexpressing cell line is a powerful tool for CRISPR interference (CRISPRi), enabling targeted gene silencing without altering the genome. By fusing nuclease-inactive Cas9 (dCas9) with the KRAB repression domain, this system suppresses transcription through epigenetic mechanisms. It is widely used for functional genomics, loss-of-function studies, and disease modeling.

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